Abstract

In the context of the growing use of biofungicides, this project aims to characterize the tolerance acquisition of aquatic biofilms (periphyton and leaf litter) toward these emerging chemicals in comparison to synthetic ones. To this end, we will implement an untargeted metabolomics approach based on high resolution mass spectrometry allowing the integrative characterization of the exposure and the global molecular/biochemical response of the whole biofilm. Also, its use in parallel of usual structural and functional descriptors will support understanding of the mechanism/responses involved in the tolerance and its ecosystem consequences (ecosystem services), and the identification of molecular markers linked to these responses. To do so, exposure in controlled conditions to model (bio)-fungicides) will be implemented in order to first unravel the mechanisms of tolerance and then evaluated the influence of environmental confounding factors. Beyond the response of the whole biofilms, the response of their individual component (as mono-specific culture and co-culture) will be investigated to order to gain understanding of the contribution of microbial interaction and species acclimatation in the acquisition of tolerance. This is a Quarto website made with the inrae-project-website extension.

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